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GATC Biotech
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Microsynth ag
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PrimerDesign Inc
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Image Search Results
Journal: Stem Cells International
Article Title: Nonviral Gene Delivery of Growth and Differentiation Factor 5 to Human Mesenchymal Stem Cells Injected into a 3D Bovine Intervertebral Disc Organ Culture System
doi: 10.1155/2013/326828
Figure Lengend Snippet: (a) and (b) pmaxGFP-transfected hMSC 48 hours after C-17 (a) or U-23 (b) electroporation using the Amaxa nucleofector. (c) and (d) GDF5 expression of transfected and control hMSC after 14 days of monolayer culture, anti-GDF5 antibody from GeneTex. Blue: cell nucleus stained by DAPI, green: translated GFP, red: intracellular GDF5. (c) Cell population at a resolution of 20x or (d) close-up (68x) hMSC transfected with RG207105, inlet in C = sham control.
Article Snippet: The ORF of the sequence of GDF5 was back-checked for accumulation of point mutations resulting from cloning using direct DNA sequencing of the insert of
Techniques: Transfection, Electroporation, Expressing, Control, Staining
Journal: Stem Cells International
Article Title: Nonviral Gene Delivery of Growth and Differentiation Factor 5 to Human Mesenchymal Stem Cells Injected into a 3D Bovine Intervertebral Disc Organ Culture System
doi: 10.1155/2013/326828
Figure Lengend Snippet: Glycosaminoglycan (GAG)/DNA ratio of outer and inner annulus fibrosus (AF) after 7 days of free-swelling organ culture of bovine intervertebral discs. Please note the difference between untransfected hMSC, control, pmaxGFP, and RG207105 transfected cells. The GDF5 overexpressing cells are pushing partially GAG/DNA ratio back in bovine IVD organ culture.
Article Snippet: The ORF of the sequence of GDF5 was back-checked for accumulation of point mutations resulting from cloning using direct DNA sequencing of the insert of
Techniques: Organ Culture, Control, Transfection